Crystallization and preliminary X-ray diffraction studies of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis

Authors
Singleton, MR Isupov, MN Littlechild, JA
Citation
Mr. Singleton et al., Crystallization and preliminary X-ray diffraction studies of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis, ACT CRYST D, 55, 1999, pp. 702-703
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
3
Pages
702 - 703
Database
ISI
SICI code
0907-4449(199903)55:<702:CAPXDS>2.0.ZU;2-T
Abstract
Pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermoco ccus litoralis has been crystallized in a form suitable for X-ray diffracti on from ammonium sulfate or ammonium dihydrogen orthophosphate using the va pour-phase diffusion method. Crystals from both precipitants are of the ort horhombic space group P2(1)2(1)2 with unit-cell dimensions a = 94.06, b = 1 49.06, c 73.54 Angstrom. A complete data set to 2.5 Angstrom resolution has been collected from crystals grown from ammonium sulfate.