Postembryonic neural proliferation in the zerbrafish forebrain and its relationship to prosomeric domains

Large gaps of knowledge exist regarding postembryonic brain morphogenesis o f the zebrafish Danio rerio (Cyprinidae, Teleostei). The zebrafish represen ts together with the frog (Xenopus), chick and mouse - one of four major mo dels for the genetic study of early brain development. Here, we used normal silver-stained Bodian material and immunohistochemical material stained wi th a monoclonal antibody against the proliferating cell nuclear antigen (PC NA, cyclin) to study the morphogenetic appearance and location of prolifera tion zones of the zebrafish brain between day 1 and day 10, focussing on th e forebrain at day 5 postfertilization. Our results directly demonstrate th at the dorsal telencephalic proliferation zone (i.e. the pallium) extends - consistent with the process of eversion - some distance laterally on top o f the telencephalon. The subpallial telencephalic proliferation consists of dorsal and ventral zones. The preoptic region also includes dorsal and ven tral proliferation zones. In the diencephalon proper, separate proliferatio n zones are present in the habenula, and in the periventricular cell masses of the dorsal thalamus, the ventral thalamus, and the pretectum. More vent rocaudally, the latter three massive proliferation zones appear to be repla ced each by thinner, but distinct proliferation zones. Two of them represen t ventrocaudal continuations of the dorsal and ventral thalamus and lie in the region referred to as the posterior tubercular area in adult teleostean neuroanatomy. The third lies in the region of the nucleus of the medial lo ngitudinal fascicle. In addition, several hypothalamic proliferation zones are present. The data for the diencephalon are largely in agreement with th e neuromeric model of brain organization of Puelles and Rubenstein (1993), which is mostly based on amniote data. Generally, the understanding of the prosomeric origin of teleostean prosencephalic cell masses may be regarded as pivotal for their comparative interpretation.