Artificial spawning of European catfish, Silurus glanis L: stimulation of ovulation using LHRH-a, Ovaprim and carp pituitary extract

Ovulation was stimulated in four groups of European catfish, Silurus glanis L., using injections of des-Gly(10), [D-Ala(6)]-LHRH Ethylamide (20 mu g k g(-1)) and pimozide (10 mg kg(-1)), Ovaprim (0.33 mL kg(-1)), and carp pitu itary extract (4 mg kg-l, in one or two doses), A higher percentage of ovul ating females (producing eggs of sufficient quality) was found after the LH RH-a and Ovaprim treatments (100% and 80%) in relation to fish treated with the pituitary extract (60% and 66.67%). The greatest weight of eggs was ob tained in the case of repeated hypophysation and LHRH-a (1299.69 and 1298.5 7 g, respectively), and the smallest after single hypophysation (1144.08 g) . After 60 h of incubation, the best quality of eggs was found in the group treated with Ovaprim (62.9% of live embryos) and the poorest in the two gr oups which underwent hypophysation (50.41% and 50.75%). No statistically si gnificant effect by the ovulation stimulators on the characteristic qualita tive and quantitative traits of obtained eggs was ascertained.