Characterization of human skin-derived CD1a-positive lymph cells

Citation
Cu. Brand et al., Characterization of human skin-derived CD1a-positive lymph cells, ARCH DERM R, 291(2-3), 1999, pp. 65-72
Citations number
45
Categorie Soggetti
da verificare
Journal title
ARCHIVES OF DERMATOLOGICAL RESEARCH
ISSN journal
03403696 → ACNP
Volume
291
Issue
2-3
Year of publication
1999
Pages
65 - 72
Database
ISI
SICI code
0340-3696(199902/03)291:2-3<65:COHSCL>2.0.ZU;2-Y
Abstract
The phenotype and function of CD1a(+) lymph cells is of considerable intere st. By means of microsurgical lymph cannulation human lymph derived from no rmal skin was sampled. Cells were isolated and processed for immunocytochem istry, electron microscopy, flow cytometry and functional assays. The major ity of the cells, (62%), were T cells. The other cells comprised CD1a(+) ce lls (7%), monocytes/macrophages (8%), and B cells (1%); the remainder were erythrocytes or uncharacterized cells. The CD1a(+) cells reacted with antib odies against protein S-100, HLA-DR, the Lag antigen, CD4, CD11a, CD11b, CD 18, CD25, CD40, CD54, CD80 and CD86. Interestingly, a small prolow portion the of CD1a(+) cells (about 5%) reacted with an antibody to CD14. The CD1a( +) cells did not react with an antibody against human follicular dendritic cells nor were they CD19-, CD23-, E-cadherin- or factor XIIIa-positive. Bot h allogenic and antigen-specific T cell proliferation stimulated by antigen -presenting lymph cells were strongly inhibited by adding anti-CD80 and ant i-CD86 antibodies. By electron microscopy Birbeck granules were detected in only 22% of the CD1a(+) lymph cells and these cells exhibited an extensive ruffling of the surface. These findings demonstrate that CD1a(+) lymph cel ls, which do not express the dermal dendritic cell marker factor XIIIa, res emble dendritic cells formerly designated as 'veiled' as well as lymphoid d endritic cells, suggesting that after migration to the regional lymphoid or gans, Langerhans cells form a more differentiated population of dendritic c ells specialized in sensitizing T lymphocytes. Our results add further supp ort to the view that resident Langerhans cells may be precursors of lymphoi d dendritic cells acquiring the final phenotype in the microenvironment of the lymph node.