Quantitative changes in maize cytoplasmic proteins induced by aluminium

Three-day-old maize seedlings were subjected to 100 mu M AlCl3 for 24 h, Cy toplasmic proteins were isolated from root tips, root base and from coleopt iles, After fractionation of cytoplasmic proteins on anion chromatography c olumn Bio-Scale Q2 sodium dodecylsulphate polyacrylamide gel electrophoresi s (SDS-PAGE) analysis was used to monitor Al-induced changes in polypeptide composition of particular fractions. Four (root) and 7 (coleoptile) fracti ons were eluted from the column with linear 0 - 1.0 M NaCl gradient. In fra ction 1 of cytoplasmic proteins from root tips Al induced accumulation of p olypeptide with molecular mass of 16 kD and simultaneous reduction of two p olypeptides (67.5 and 60 kD), In fraction 1 isolated from mature zone of ma ize roots Al-induced accumulation of 22 kD polypeptide and reduction of 67. 5, 60, and 14 kD polypeptides. Most pronounced changes were revealed in col eoptile. In three protein fractions increased accumulation of polypeptides with molecular mass of 14, 17.5, 20, 24.5, 28, 30, and 37.5 kD were observe d, In the remaining three root or four coleoptile fractions of cytoplasmic proteins, no differences were found between A1-treated and control maize se edlings.