Efficient synthesis of 3-aminodigoxigenin and 3-aminodigitoxigenin probes

Oxidation of digoxigenin and digitoxigenin to the 3-ketones followed by red uctive amination produced a mixture of amine epimers. The inability to sepa rate the epimeric mixtures of chemiluminescent digoxigenin probes derived b y conjugation to the acridinium label prompted us to develop an HPLC method to separate the amines. Labeling of the pure amines resulted in good yield s of the isomerically pure probes. (C) 1999 Elsevier Science Ltd. All right s reserved.