Analysis of peptides from animal and plant tissues

A new technique was developed for the analysis of peptide compositions of e xtracts from various animal and plant tissues, It involves the acidic extra ction of a peptide fraction from the starling material and its precipitatio n with acetone, fractionation of peptides by ion-exchange chromatography by using a stepwise elution of fractions and detection by means of ninhydrin color reaction, and computer processing of the results. For the presentatio n of the results of analysis, chromatographic profiles and peptidograms wer e proposed, The results of analysis can be stored in a database and used fo r the creation of "generalized peptide portraits" and "differential peptide portraits" of the subjects investigated, which allow the identification of peptides characteristic of the subjects, The amount of peptide undergoing analysis ranged from 1 to 10 nmol.