Kinetic modeling of oligosaccharide synthesis catalyzed by Leuconostoc mesenteroides NRRL B-1299 dextransucrase

The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as subs trate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B -512F dextransucrase. There are significant correlations between the parame ters of the soluble form of B-1299 dextransucrase and those calculated for the B-512F enzyme; that is, their properties are comparable and differ from those of the insoluble form of B-1299 dextransucrase. Whereas the calculat ed parameters for high maltose concentrations describe the kinetic behavior very well, the time curves for low maltose concentrations were not describ ed correctly. Therefore, the parameters were calculated separately for the two ranges. (C) 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 308-315, 1999.