M. Mengozzi et al., Human immunodeficiency virus replication induces monocyte chemotactic protein-1 in human macrophages and U937 promonocytic cells, BLOOD, 93(6), 1999, pp. 1851-1857
We have recently described a significant correlation between human immunode
ficiency virus-1 (HIV-1) RNA replication and monocyte chemotactic protein-1
(MCP-1) levels in the cerebrospinal fluid (CSF) of individuals with the ac
quired immunodeficiency syndrome (AIDS) with HIV encephalitis (E). Because
local macrophages (microglia) are the cells predominantly infected in the b
rain, we investigated whether in vitro HIV infection affects MCP-1 producti
on in mononuclear phagocytes (MP). MCP-1 secretion and expression were cons
instently upregulated over constitutive levels in human monocyte-derived ma
crophages (MDM) infected with the M-tropic R5 Bat strain of HIV-1. HIV repl
ication was required for this effect, as demonstrated by the absence of che
mokine upregulation after infection in the presence of 3'-azido-3'-deoxythi
midine (AZT) or cell-exposure to heat-inactivated (Delta degrees) virus. MC
P-1 induction was not restricted to HIV-1 Bat, but was also observed during
productive infection of MDM with two primary isolates differing for entry
coreceptor usage and of U937 cells with the X4 HIV-1 MN strain. Based on th
e observation that exogenous HIV-1 Tat induced MCP-1 expression in astrocyt
es, we also investigated its role in MDM and U937 cells. Exogenous Tat indu
ced MCP-1 production from MDM in a concentration-dependent manner, however,
it was not effective on uninfected U937 cells or on the chronically infect
ed U937-derived cell line U1. Transfection of Tat-expressing plasmids moder
ately activated HIV expression in U1 cells, but failed to induce MCP-1 expr
ession in this cell line or in uninfected U937 cells. HIV replication-depen
dent expression of MCP-1 in MP may be of particular relevance for the patho
genesis of HIV infection in nonlymphoid organs such as the brain. (C) 1999
by The American Society of Hematology.