INSERTIONALLY INACTIVATED AND INDUCIBLE RECA ALLELES FOR USE IN NEISSERIA

Authors
SEIFERT HS
Citation
Hs. Seifert, INSERTIONALLY INACTIVATED AND INDUCIBLE RECA ALLELES FOR USE IN NEISSERIA, Gene, 188(2), 1997, pp. 215-220
Citations number
22
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
188
Issue
2
Year of publication
1997
Pages
215 - 220
Database
ISI
SICI code
0378-1119(1997)188:2<215:IIAIRA>2.0.ZU;2-F
Abstract
Two classes of recA mutations have been constructed for use in Neisser ia gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec (-) and Rec(+) by the removal or addition of IPTG. The effects of regu lating recA expression on the processes of DNA transformation, DNA rep air and pilin-phase variation are described. These regulatable cassett es can also be used to control the expression of any chromosomal gene.