Aberrant expression of growth factor Wnt-5A in six urinary malignant cell lines

Objective To investigate the expression of growth factor Wnt-5A in urinary tumor cell lines. Methods By semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt-5A in six urinary malignant cell lines, o ne primary cultured renal fibroblast and one case of normal human renal tis sue was detected using Gel Doc 1000 computer controlled molecular analysis system. Results The expression of Wnt-5A in urinary tumor cell lines was much highe r than that in primary cultured renal fibroblasts (PRF) and normal human re nal tissue (NRT). The levels of Wnt-5A mRNA were different between six mali gnant cell lines. Conclusion The overexpression of growth factor Wnt-5A has a potential effec t on the oncogenesis of urinary malignancies.