Objective To investigate the expression of growth factor Wnt-5A in urinary
tumor cell lines.
Methods By semi-quantitative RT-PCR (reverse transcriptase polymerase chain
reaction), the expression of Wnt-5A in six urinary malignant cell lines, o
ne primary cultured renal fibroblast and one case of normal human renal tis
sue was detected using Gel Doc 1000 computer controlled molecular analysis
system.
Results The expression of Wnt-5A in urinary tumor cell lines was much highe
r than that in primary cultured renal fibroblasts (PRF) and normal human re
nal tissue (NRT). The levels of Wnt-5A mRNA were different between six mali
gnant cell lines.
Conclusion The overexpression of growth factor Wnt-5A has a potential effec
t on the oncogenesis of urinary malignancies.