High-performance liquid chromatographic method for separation and quantification of intact glucosinolates

A simple, economical and efficient HPLC method has been developed for the s eparation and determination of the individual glucosinolates in rapeseed an d mustard. The method involves single-step extraction of glucosinolates wit h boiling water and separation of the individual glucosinolates on a Novapa ck RP-18 column (3.9 mm x 150 mm) with 0.2 M ammonium sulphate as mobile ph ase. Peaks were monitored at 229 nm. All major glucosinolates could be elut ed within 10 min. The method proved effective for routine analysis of gluco sinolates.