Isolation of human lymphocyte antigens class I-restricted cytotoxic T lymphocytes against autologous myeloma cells

Peripheral blood T cells from a patient with multiple myeloma in complete r emission were selected in vitro against an autologous myeloma cell line (SB N-1), using a protocol designed for the selection of relatively rare precur sor cytotoxic T cells (pCTL). Delayed addition (2 weeks) of interleukin 2 i nduced T-cell proliferation, and a bulk culture (T-cell line) was obtained 2 days later. This T-cell line displayed cytotoxicity against SBN-1, A CD8( +) CD4(-) cytotoxic T-cell clone (CT5) was then obtained that recognized SB N-1 but not autologous EBV + B-lymphoblastoid cells, autologous T PHA-blast s, or Daudi, Raji, K562, and 11 allogeneic myeloma cell lines, Moreover, CT 5 cytotoxic activity against SBN-1 was blocked by monoclonal antibodies rec ognizing human lymphocyte antigen class I molecules. This seems to be the f irst demonstration of myeloma-specific pCTL in peripheral blood T cells of patients with multiple myeloma.