K. Ebner et al., Vasopressin released within the septal brain area during swim stress modulates the behavioural stress response in rats, EUR J NEURO, 11(3), 1999, pp. 997-1002
The aim of the present study was to investigate the physiological significa
nce of the neuropeptide arginine vasopressin (AVP) released within the sept
um, in the behavioural response of rats to stress. In the first experiment,
rats were chronically implanted with a microdialysis probe aimed at the me
diolateral or ventral septum to monitor the local release of AVP in respons
e to 10 min of forced swimming in 20 degrees C warm water. Exposure to this
stressor caused a significant increase in AVP release in both the mediolat
eral (174 +/- 21%, P < 0.01) and ventral septum (220 +/- 33%, P < 0.01). In
contrast, microdialysates collected outside the mediolateral septum or in
the lateral ventricle remained at prestress levels throughout the dialysis
period. Furthermore, unstressed control animals failed to show significant
alterations in vasopressin release in the mediolateral septum. In a second
experiment, the introduction of the V1 receptor antagonist d(CH2)(5)Tyr(Me)
AVP into the mediolateral septum via inverse microdialysis concomitant with
stressor exposure caused the rats to spend an increased time floating and
a reduced time swimming compared to vehicle-treated rats. This effect was a
cute and also detected 24 h after antagonist administration. Taken together
, these findings demonstrate a significant activation of the septal vasopre
ssinergic system in response to swim stress. Furthermore, our data support
the view that AVP released within this brain area is involved in the genera
tion of active behavioural strategies aimed at coping with new and challeng
ing situations.