Citation
Dp. Kharakoz et al., Stoichiometry of dipalmitoylphosphatidylcholine-DNA interaction in the presence of Ca2+: a temperature-scanning ultrasonic study, FEBS LETTER, 446(1), 1999, pp. 27-29
Citations number
16
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793
→ ACNP
Volume
446
Issue
1
Year of publication
1999
Pages
27 - 29
Database
ISI
SICI code
0014-5793(19990305)446:1<27:SODIIT>2.0.ZU;2-U
Abstract
DNA-DPPC complexes can be prepared by means of a single step procedure of m
ixing DNA solution and aqueous lipid dispersion in the presence of calcium
ions. Interaction between DPPC and DNA brings about a biphasic shape of mel
ting curves corresponding to the free lipid and the strongly bound one. The
amount of the strongly bound lipid is 5 molecules per nucleotide which is
close to the size of the first lipid monolayer around DNA molecule. (C) 199
9 Federation of European Biochemical Societies.