Multiple regulatory elements control the expression of the yeast ACR1 gene

The ACR1 gene, encoding a succinate-fumarate transporter, is required by th e yeast Saccharomyces cerevisiae for ethanol utilization. Accordingly, gene expression is induced by ethanol and repressed by glucose. Here, we invest igated three carbon source response elements present in its promoter region . Specific deletions as well as functional analysis of the elements in a he terologous promoter confirmed their role in transcriptional regulation. Pro tein binding to carbon source response elements of the ICL1 promoter was co mpeted by all three elements to various extents by the respective ACR1 sequ ences. In addition, two putative stress response promoter elements present in the ACR1 promoter were investigated in deletion analyses and shown to co ntribute to gene expression. (C) 1999 Federation of European Biochemical So cieties.