L. Daulhac et al., Gastrin stimulates the formation of a p60(Src)/p125(FAK) complex upstream of the phosphatidylinositol 3-kinase signaling pathway, FEBS LETTER, 445(2-3), 1999, pp. 251-255
The molecular events whereby gastrin occupancy of G/CCKB receptors leads to
phosphatidylinositol (PI) 3-kinase activation have been examined, We repor
t here that this peptide promotes the association between two non-receptor
tyrosine kinases, p60(Src) and p125(FAK), and elicits a parallel increase i
n tyrosine phosphorylation and activity of both kinases. Gastrin-induced PI
3-kinase activity was coprecipitated with p60(Src) and p125(FAK) and was i
nhibited by herbimycin A, the selective Src inhibitor PP-2 or cytochalasin
D, which disrupts the actin cytoskeleton and prevents p125(FAK) activity. T
hese results indicate, for the first time, that a p60(Src)/p125(FAK) comple
x acts upstream of the gastrin-stimulated PI 3-kinase pathway, (C) 1999 Fed
eration of European Biochemical Societies.