Within an EC-study of the relationship between the chemistry of the aquatic
system and resulting toxicological effects of heavy metals on micro-organi
sms, the speciation of trace metals is being assessed. The set-up of a spec
iation scheme is described, in which surface water is characterised in term
s of total metal concentrations, nutrients, general parameters like DOG, pH
and conductivity, metal binding properties and in which biologically relev
ant metal fractions are determined.