Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase

Citation
Le. Brennan et al., Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase, GENE, 228(1-2), 1999, pp. 85-91
Citations number
30
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
03781119 → ACNP
Volume
228
Issue
1-2
Year of publication
1999
Pages
85 - 91
Database
ISI
SICI code
0378-1119(19990304)228:1-2<85:CAMLOA>2.0.ZU;2-3
Abstract
AU-rich elements function as instability elements which direct rapid mRNA d egradation. AUH protein exhibits an AU-specific RNA-binding property and an intrinsic enoyl-CoA hydratase activity and may therefore function to link mRNA decay to metabolic processes (Nakagawa et al., 1995. Proc. Natl. Acad. Sci. USA 92, 2051-2055). The sequence encoding the murine protein, muAUH, was established by cloning, and the corresponding polypeptide predicted to have a molecular mass of 37 kDa. As shown for the human protein, muAUH is e xpressed in a 32 kDa form and there is 94% homology between the two species . Recombinant muAUH was shown to be an RNA-binding enoyl-CoA hydratase. All murine cells studied contained a single AUH transcript of approx. 1.7 kb a nd an investigation of tissue-specific expression revealed highest levels i n kidney, skeletal muscle, heart, brain and spleen. It was further determin ed, using immunoelectron microscopy, that AUH is located in the mitochondri a of mouse cells. (C) 1999 Elsevier Science B.V. All rights reserved.