Detection of Tropheryma whippelii DNA (Whipple's disease) in faeces

To date the diagnosis of Whipple's disease is based mainly on the histopath ological analysis of duodenal biopsies since Tropheryma whippelii cannot be cultured in vitro. We investigated the possibility to diagnose Whipple's d isease by detection of bacterial DNA in faeces. Nested polymerase chain rea ction with amplification of part of the 16S rRNA gene of this bacterium in DNA extracted from faeces of-a patient with Whipple's disease was performed . Sequencing of the polymerase chain reaction product revealed the sequence of Tropheryma whippelii. We conclude that Whipple's disease will be able t o be diagnosed non-invasively by DNA analysis from the faeces as soon as mo re specific sequences of this bacteria are known.