Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi: Relations with culturable fungi, reported home dampness, and respiratory symptoms

Background: Epidemiologic studies have demonstrated an association between indoor fungal growth and respiratory symptoms. However, in only a few studi es was fungal exposure actually measured. Objective: The purpose of this study was to evaluate the measurement by enz yme immunoassay of extracellular polysaccharides of Aspergillus and Penicil lium species (EPS-Asp/Pen) in house dust as a marker for fungal exposure an d to study the relations between EPS-Asp/Pen levels and home dampness and r espiratory symptoms in children. Methods: Extracts of house dust samples from bedroom and living room floors and mattresses from homes of 31 children with chronic respiratory symptoms and 29 children with no chronic respiratory symptoms were analyzed for EPS -Asp/Pen. Results: EPS-Asp/Pen were readily detectable (40 to 46,513 nanogram equival ent/g dust) in 161 house dust extracts, with highest concentrations in livi ng room Boor dust. EPS-Asp/Pen levels were 2 to 3 times higher on carpeted floors than on smooth floors. EPS-Asp/Pen were significantly correlated wit h total culturable fungi (r = 0.3 to 0.5) and with house dust mite allergen s (r = 0.3 to 0.5). EPS-Asp/Pen levels in living room floor dust were posit ively associated with occupant-reported home dampness. This was not observe d for EPS-Asp/Pen in bedroom floor and mattress dust. EPS-Asp/Pen levels in living room Boor dust were positively associated with respiratory symptoms , EPS-Asp/Pen in bedroom floor and mattress dust showed a reversed associat ion with respiratory symptoms, possibly because of allergen-avoidance measu res taken in the bedroom. Conclusion: The enzyme immunoassay for fungal EPS-Asp/Pen may be a useful m ethod for exposure assessment of indoor fungi.