A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant

Authors
Heath, RJ Rock, CO
Citation
Rj. Heath et Co. Rock, A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant, J BACT, 181(6), 1999, pp. 1944-1946
Citations number
15
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
6
Year of publication
1999
Pages
1944 - 1946
Database
ISI
SICI code
0021-9193(199903)181:6<1944:AMMAFT>2.0.ZU;2-5
Abstract
The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein ha d a significantly reduced glycerol-3-phosphate acyltransferase specific act ivity coupled with an elevated K-m for glycerol-3-phosphate.