MOLECULAR IMPRINTING OF ACETYLATED CARBOHYDRATE-DERIVATIVES INTO METHACRYLIC POLYMERS

The non-covalent imprinting procedure was used for the preparation of polymers selective for various carbohydrate derivatives, i.e. peracety lated phenyl alpha- and beta-D-glycosides of galactose. The selectivit ies of the resulting polymers were tested in a HPLC procedure. The sel ectivity was influenced by the anomeric configuration of the glycoside (alpha- or beta-configuration). Thus, a polymer prepared with p-amino phenyl beta-galactoside as print molecule; gave,a relatively high sele ctivity (alpha = 1.27) for the beta-galactoside over the alpha-galacto side, whereas a polymer prepared with the corresponding alpha-galactos ide showed no or low selectivity towards the alpha-glycoside (alpha = 1.02). Moreover, the structure of the aglycon was important. Thus, the use of aminophenyl glycosides as print molecules resulted in polymers with induced selectivity, whereas the use of the corresponding nitrop henyl- and acetaminophenyl-galactosides gave no induced selectivity.