GENETIC DIVERSITY OF BRADYRHIZOBIUM-JAPONICUM FIELD POPULATION REVEALED BY RAPD FINGERPRINTING

Citation
S. Sikora et al., GENETIC DIVERSITY OF BRADYRHIZOBIUM-JAPONICUM FIELD POPULATION REVEALED BY RAPD FINGERPRINTING, Journal of applied microbiology, 82(4), 1997, pp. 527-531
Citations number
30
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
13645072
Volume
82
Issue
4
Year of publication
1997
Pages
527 - 531
Database
ISI
SICI code
1364-5072(1997)82:4<527:GDOBFP>2.0.ZU;2-O
Abstract
RAPD fingerprinting was used for strain identification and the assessm ent of genetic diversity within a field population of Bradyrhizobium j aponicum. Total genomic DNAs from 13 field isolates and two inoculant strains were amplified using six different 10-mer primers. Different a nd informative band patterns were obtained for all strains analysed. C luster analysis unexpectedly revealed that none of the field isolates was identical to inoculant strains which were regularly used for soybe an inoculation. Among field isolates two highly divergent groups were determined. The results indicate that RAPD is a very discriminative an d efficient method for differentiating and studying genetic diversity of B. japonicum strains.