COMPREHENSIVE ONLINE RPLC-CZE-MS OF PEPTIDES

Peptide standards and tryptic digests of ribonuclease B are separated by comprehensive two-dimensional reversed phase liquid chromatography (RPLC) and capillary zone electrophoresis (CZE) and detected on-line b y electrospray mass spectrometry. The RPLC column is coupled to the CZ E column by a transverse flow gating interface. A new rugged microelec trospray needle is described that combines high ionization efficiency, low flow rates, and a sheath now. The result is a system combining th e separation capabilities of both RPLC and CZE with on-line mass spect rometric detection, all in about 15 min. (C) 1997 American Society for Mass Spectrometry.