QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIPS OF ACYCLOVIR ESTERS USING IMMOBILIZED ALBUMIN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Citation
Ds. Ashton et al., QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIPS OF ACYCLOVIR ESTERS USING IMMOBILIZED ALBUMIN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 707(2), 1995, pp. 367-372
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
707
Issue
2
Year of publication
1995
Pages
367 - 372
Database
ISI
SICI code
Abstract
Acyclovir and 18 of its esters have been investigated by systematic me asurement of their reversed-phase high-performance liquid chromatograp hic retention using differing mobile phase compositions. The methanol content of the mobile phase was varied between 5 and 95%. By linear le ast squares regression of the logarithmic retention factor (log k') ag ainst methanol concentration, the slope (S) and intercept (log k'(0)) of the so obtained straight lines were calculated for each compound. T he chromatographic hydrophobicity index (phi(0,MeOH)) calculated from the S and log k'(0) values (phi(0,MeOH) = - log k'(0)/S) showed signif icant correlation (r > 0.96) to the calculated octanol-water partition coefficients (clog P). The albumin-binding properties of the compound s were characterised by the log k'(HSA) values obtained by using an im mobilised human serum albumin (HSA) HPLC column and 1% propan-2-ol 99% aqueous 10 mM phosphate buffer pH 7.0 as mobile phase. The measured a lbumin-binding parameters showed significant correlations to the clog P,phi(0,MeOH), S and log k'(0) values, establishing the importance of hydrophobic properties to the interaction of the acyclovir derivatives with HSA.