QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIPS OF ACYCLOVIR ESTERS USING IMMOBILIZED ALBUMIN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Ds. Ashton et al., QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIPS OF ACYCLOVIR ESTERS USING IMMOBILIZED ALBUMIN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 707(2), 1995, pp. 367-372
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Acyclovir and 18 of its esters have been investigated by systematic me
asurement of their reversed-phase high-performance liquid chromatograp
hic retention using differing mobile phase compositions. The methanol
content of the mobile phase was varied between 5 and 95%. By linear le
ast squares regression of the logarithmic retention factor (log k') ag
ainst methanol concentration, the slope (S) and intercept (log k'(0))
of the so obtained straight lines were calculated for each compound. T
he chromatographic hydrophobicity index (phi(0,MeOH)) calculated from
the S and log k'(0) values (phi(0,MeOH) = - log k'(0)/S) showed signif
icant correlation (r > 0.96) to the calculated octanol-water partition
coefficients (clog P). The albumin-binding properties of the compound
s were characterised by the log k'(HSA) values obtained by using an im
mobilised human serum albumin (HSA) HPLC column and 1% propan-2-ol 99%
aqueous 10 mM phosphate buffer pH 7.0 as mobile phase. The measured a
lbumin-binding parameters showed significant correlations to the clog
P,phi(0,MeOH), S and log k'(0) values, establishing the importance of
hydrophobic properties to the interaction of the acyclovir derivatives
with HSA.