Isolation of a cDNA encoding active protein for kiwifruit ACC synthase andits specific expression in the outer pericarp

Authors
Ikoma, Y Yano, M Xu, ZC Ogawa, K
Citation
Y. Ikoma et al., Isolation of a cDNA encoding active protein for kiwifruit ACC synthase andits specific expression in the outer pericarp, J JPN S HOR, 68(2), 1999, pp. 286-288
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
ISSN journal
00137626 → ACNP
Volume
68
Issue
2
Year of publication
1999
Pages
286 - 288
Database
ISI
SICI code
0013-7626(199903)68:2<286:IOACEA>2.0.ZU;2-#
Abstract
ACC (1- aminocyclopropane- 1-carboxylate) synthase cDNA (AD-ACS1), isolated from kiwifruit 'Hayward', was expressed as a fusion protein coupled with g lutathione S-transferase (GST) in Escherichia coli. The fusion protein exhi bited ACC synthase activity. Northern blot analyses showed that the transcr ipts hybridized with AD-ACS1 accumulated only in the outer pericarp after a 24- hr ethylene treatment.