M. Matsuta et al., DETECTION OF NUMERICAL CHROMOSOMAL-ABERRATIONS IN MALIGNANT MELANOMASUSING FLUORESCENCE IN-SITU HYBRIDIZATION, Journal of cutaneous pathology, 24(4), 1997, pp. 201-205
To evaluate the numerical chromosomal aberration in malignant melanoma
, we have applied fluorescence in situ hybridization (FISH) with repet
itive DNA probes specific for chromosomes 1, 6, 7, 9, 10, and 17 on 24
fresh malignant melanomas (primary: 14, metastatic: 8). We defined a
tumor that had copies with more than 3 spots as chromosomal gain. Chro
mosomal copy number gain was found in 40.9% of the cases for chromosom
e 7, 27.2% for chromosome 6, 27.2% for chromosome 17, 22.7% for chromo
some 9 and 10, and 4.5% for chromosome 1. Monosomy was found in 54.5%
of the cases for chromosome 10, 36.5% for chromosome 9, 27.2% for chro
mosome 6, 22.7% for chromosome 17, and 18.1% for chromosome 1 and 7. T
he most frequent numerical alterations were seen in chromosomes 6, 7,
9 and 10. Gain of chromosome 6 and 7 and/or losses of chromosome 9 and
10 may play an important role in the tumorigenesis and development of
malignant melanomas.