F. Carimi et al., Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group [Citrus sinensis (L.) Osb.], PL CELL TIS, 54(3), 1998, pp. 183-189
Somatic embryogenesis was induced and plant regeneration was obtained in 11
different genotypes of sweet orange navel group [Citrus sinensis (L.) Osb.
] from cultures of stigma/style explants and underdeveloped ovules. Explant
s were cultured on 3 different modifications of Murashige and Skoog medium:
500 mg 1(-1) malt extract; 500 mg 1(-1) malt extract and 4.6 mu M kinetin;
and 500 mg 1(-1) malt extract and 13.3 mu M 6-benzylaminopurine. Sucrose (
146 mM) was used as carbon source. Somatic embryogenesis occurred 1-3 month
s after culture initiation from undeveloped ovule and stigma/style cultures
of all the genotypes tested. Somatic embryos developed into plantlets with
a high frequency (74%) after transfer to Murashige and Skoog medium supple
mented with 146 mM sucrose and 500 mg 1(-1) malt extract. Plants were succe
ssfully transferred to soil.