Rapid identification of the new anabaenopeptin G from Planktothrix agardhii HUB 011 using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Toxic water blooms from cyanobacteria in lakes and rivers are a worldwide p henomenon. A new technique is presented for the rapid detection of toxic an d nontoxic blooms. Matrix-assisted laser desorption/ionization time-of-flig ht mass spectrometry (MALDI-TOFMS) was employed to identify mainly peptide metabolites (microcystins, anabaenopeptins, cyanopeptolins, microviridins, microginins and aeruginosins) from microgram quantities of prepared cells w ithin minutes, The spectra show the presence of peptides in strains or wate r blooms simultaneously. A new compound has been identified using the post source decay (PSD) and collision induced dissociation (CID) mode. This new compound has been defined as anabaenoeptin G, The potential of the method f or screening of various secondary metabolite producers for defined products including antibiotics is discussed, Copyright (C) 1999 John Whey & Sons, L td.