Determination of anabolic steroids by gas chromatography negative-ion chemical ionization mass spectrometry and gas chromatography negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization
Mh. Choi et al., Determination of anabolic steroids by gas chromatography negative-ion chemical ionization mass spectrometry and gas chromatography negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization, RAP C MASS, 13(5), 1999, pp. 376-380
A gas chromatography/mass spectrometry (GC/MS) method is described which us
es negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/
MS) for the determination of eight anabolic steroids in human urine. Eight
anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA),
and were determined using GC/NCI-MS and GC/NCI-MS/MS. The linear correlatio
n coefficients for calibration in NCI-MS/MS were in the range 0.9880-0.9988
, This method of derivatization with HFBA for use with GC/NCI was useful in
determinations of 19-norandrosterone, boldenone, 19-noretiocholanolone, 2-
methylandrosterone, nandrolone, 1-methyleneandrosterone, 1-methylandrostero
ne, 4-dihydroboldenone and mesterolone, The detection limits of this proced
ure were 5-20 ppb at a signal-to-noise (S/N) ratio of 3, Copyright (C) 1999
John Wiley & Sons, Ltd.