Oa. Timofeeva et al., Effect of cytoskeleton modifiers on the lectin activity during plant acclimation to low temperatures and under ABA treatment, RUSS J PL P, 46(2), 1999, pp. 147-151
Effects of inhibitors of microtubule polymerization, 1 mM colchicine and 15
mu M oryzalin, and a microtubule stabilizer, 7% dimethyl sulfoxide, on the
activities of soluble and cell-wall lectins were studied in unhardened (23
degrees C) and cold acclimated (3-7 days at 2-3 degrees C) winter wheat (T
riticum aestivum L,.) 7-day-old seedlings. Three wheat cultivars differing
in their frost tolerance were compared. Seedlings were grown in the presenc
e of 30 mu M ABA or without hormone in the medium. Cell-wall lectins were m
ore sensitive to the cytoskeleton modifiers than soluble lectins. Colchicin
e and oryzalin enhanced lectin activity, whereas dimethyl sulfoxide suppres
sed it. Plant hardening and ABA treatment reduced the sensitivity of cell-w
all lectins to the cytoskeleton inhibitors, probably due to appearance of t
he subpopulation of cold-stable microtubules. An increase in the activity o
f cell-wall lectins under the conditions resulting in the cytoskeleton degr
adation could be a compensatory mechanism directed to cytoskeleton stabiliz
ation, with genotype-dependent manifestation of this mechanism.