Antibody networks for surface plasmon resonance immunosensors

Citation
E. Brynda et al., Antibody networks for surface plasmon resonance immunosensors, SENS ACTU-B, 54(1-2), 1999, pp. 132-136
Citations number
9
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences","Instrumentation & Measurement
Journal title
SENSORS AND ACTUATORS B-CHEMICAL
ISSN journal
09254005 → ACNP
Volume
54
Issue
1-2
Year of publication
1999
Pages
132 - 136
Database
ISI
SICI code
0925-4005(19990125)54:1-2<132:ANFSPR>2.0.ZU;2-C
Abstract
A technique for preparation of three-dimensional networks of antibodies for surface plasmon resonance biosensors is described. This technique is based on the covalent crosslinking of a multiple molecular layer of monoclonal a ntibodies prepared by alternating adsorption of molecular layers of a posit ively charged antibodies at pH below its isoelectric point and dextran sulf ate polyanion. As a model system, networks of monoclonal antibodies against human choriogonadotropin and horseradish peroxidase were prepared and thei r properties were studied using Fourier transform infrared multiple interna l reflection spectroscopy, ELISA and surface plasmon resonance spectroscopy . Experimental results indicate that a sensor employing a network of antibo dies exhibits an enhanced sensitivity to the respective antigens in compari son with those using two-dimensional monomolecular layers, mainly because o f a higher number of receptors accessible to the antigens and better bindin g properties of antibodies that are not immobilized directly on the sensor surface. (C) 1999 Elsevier Science S.A. All rights reserved.