In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

Authors
Juretschko, S Schonhuber, W Kulakauskas, S Ehrlich, DS Schleifer, KH Amann, R
Citation
S. Juretschko et al., In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II, SYST APPL M, 22(1), 1999, pp. 1-8
Citations number
52
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
07232020 → ACNP
Volume
22
Issue
1
Year of publication
1999
Pages
1 - 8
Database
ISI
SICI code
0723-2020(199902)22:1<1:ISDOEC>2.0.ZU;2-0
Abstract
A method is described for the in situ detection of individual whole fixed c ells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a t arget molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regul atory RNA I did not result in the in situ detection of plasmid-bearing cell s.