Ja. Noguiera-machado et al., Calorimetry: a highly sensitive technique for evaluating the effect of IL-2, IFN-gamma and IL-10 on the response of peripheral blood mononuclear cells, THERMOC ACT, 327(1-2), 1999, pp. 57-62
We report the application of a calorimetric method for evaluating the metab
olic response of peripheral blood mononuclear cells (PBMC) either from S. m
ansoni infected patients or from normal subjects under stimulation with ant
igen or with cytokines. Our results demonstrate that the antigenic stimulat
ion of the PBMC with soluble adult worm antigenic preparation (SWAP) was ab
le to discriminate PBMC from infected and non-infected donors, based on cal
orimetric cell reactivity. The total heat release in the stimulation of PBM
C with interleukin 2 (IL-2) or gamma interferon (IFN-gamma) was greater for
PBMC from infected patients than normal subjects. The activation of the re
lease heat in PBMC induced by IFN-gamma, IL-2 or antigen (SWAP) was inhibit
ed by pretreatment of PBMC with interleukin 10 (IL-10). However, this inhib
ition mediated by IL-10 could be promptly reversed by pretreatment of IL-10
with its respective anti-IL-10 monoclonal antibody. Our present results ex
tend the use of the calorimetric assay and suggest its application for stud
ying cellular reactivity in basic and applied immunological systems. (C) 19
99 Elsevier Science B.V. All rights reserved.