DIABETES-MELLITUS AND SUBJECTS AGING - A STUDY ON THE ATP CONTENT ANDATP-RELATED ENZYME-ACTIVITIES IN HUMAN ERYTHROCYTES

Na+/K+- and Ca2+-ATPase are the major ATP-dependent membrane-bound enz ymes that regulate the cation transmembrane gradient which is altered both in red blood cell (RBC) senescence and in RBCs of diabetic patien ts. In an attempt to clarify the possible connection between diabetes mellitus and ageing, we investigated the relationship between RBC ATP content, Na+/K+-ATPase, Ca2+-ATPase activities and ageing in healthy, insulin-dependent (IDDM) and non-insulin-dependent (NIDDM) subjects. A significant correlation was found (r = -0.82; P < 0.001) between RBC ATP content and subject's age only in the control group. A significant reduction in Na+/K+-ATPase activity was observed in the older group ( C-2) Of control subjects, in comparison with the younger (C-1) one. In both IDDM and NIDDM subjects, the enzymatic activity was significantl y decreased when compared with healthy subjects of similar age (P < 0. 001). A significant negative correlation was found between age and enz ymatic activity in healthy subjects (r = -0.60; P < 0.001). No differe nce was observed in the RBC membrane Ca2+-ATPase activity between youn ger (C-1) and older (C-2) healthy subjects. Ca2+-ATPase activity was s ignificantly increased both in IDDM patients compared with C-1 (P < 0. 001) and in NIDDM patients compared with C-2 (P < 0.001). The present data indicate that ageing causes a reduction in the erythrocyte ATP co ntent in both healthy and diabetic subjects. In diabetic patients Na+/ K+-ATPase activity decreases independently of age.