Microvessel density in prostate cancer: Lack of correlation with tumor grade, pathologic stage, and clinical outcome

Objectives. Angiogenesis is believed to play an important role in tumor pro gression and metastasis. Previous studies have suggested that the microvess el density (MVD) of prostate tumors may be of prognostic value. This study investigated the reliability of assessing MVD in radical prostatectomy spec imens and its value as an independent prognostic indicator in men with clin ically localized prostate cancer. Methods, One hundred radical prostatectomy specimens from 1993 to 1995 were randomly selected for this study. Thirteen cases were excluded because the patients had undergone neoadjuvant hormonal therapy or tissue blocks were unavailable. The median follow-up time was 36 months. Tumor blocks were imm unostained using the endothelial-specific antibody CD31. MVD was counted in areas with the greatest microvessel immunostaining, which were designated "hot spots." MVD was analyzed for associations with clinical and pathologic factors. In a subset of 60 cases, the same observer repeated the counts th ree times. Results. Intraobserver reliability for MVD counting was excellent (reliabil ity coefficient: 0.82), demonstrating that this method could be reproduced by a single observer. MVD was not associated with Gleason sum, tumor stage, surgical margin status, or seminal vesicle invasion. Of the 87 patients, 2 0 (23%) had a prostate-specific antigen (PSA) failure during a 36-month med ian follow-up time. As expected, Gleason sum and tumor stage were strong pr edictors of PSA failure, with risk ratios of 2.1 and 2.3, respectively. In contrast, MVD was not associated with PSA failure. Conclusions. MVD, as determined by CD31, can be reliably measured by a sing le observer, but it is not a useful prognostic indicator for men with clini cally localized prostate cancer. (C) 1999, Elsevier Science Inc. AII rights reserved.