Tr. Hashimoto et al., Effects of estrogen and progesterone on urinary bladder in female rabbit: Evaluation by quantitative morphometric analyses, UROLOGY, 53(3), 1999, pp. 642-646
Objectives. To investigate possible effects of estrogen and/or progesterone
on the histologic characteristics of female rabbit urinary bladders, we ca
rried out quantitative morphometric analysis of the rabbit bladders.
Methods, Mature female rabbits were treated by ovariectomy with and without
successive estrogen and/or progesterone administration, Area densities of
the connective tissue (CT) and smooth muscle (SM) cells, the area of single
SM cells, and the thickness of the bladder wall were determined by compute
r-assisted quantitative morphometric analysis.
Results. Six weeks after each treatment, ovariectomy alone resulted in a de
crease in CT density of the bladder. Successive estrogen treatment increase
d the bladder wet weight and SM cell density within the bladder wall. Proge
sterone treatment reduced CT degradation in ovariectomized rabbits. Sex ste
roids did not significantly influence the area of each SM cell. There was n
o significant difference in histologic characteristics between the rabbits
treated by estrogen alone and those treated by combination (estrogen and pr
ogesterone) therapy.
Conclusions. Ovariectomy and successive hormonal replacement therapy result
ed in morphologic changes within the rabbit urinary bladder. Cotreatment wi
th progesterone did not significantly change the morphologic findings produ
ced by estrogen treatment alone. (C) 1999, Elsevier Science Inc. All rights
reserved.