Construction and murine immunogenicity of recombinant Bacille Calmette Guerin vaccines expressing the B subunit of Escherichia coli heat labile enterotoxin

Three recombinant strains of Mycobacterium bovis Bacille Calmette Guerin (r BCG) were prepared in which the immunogenic B subunit of human Escherichia coli heat labile enterotoxin (LT-Bh) was expressed either as a cytoplasm pr otein, a cell wall associated lipoprotein or a secreted protein. Intraperit oneal immunisation of mice with these rBCG induced IgG and IgA antibodies t o LT-Bh and shifted the serum Ige subclass response to subsequent challenge with purified LT-Bh from IgG(1) to an IgG(2a). Oral administration of reco mbinant BCG induced mucosal and serum IgA antibodies to LT-Bh which peaked four months after immunisation. Antibody responses were greater when LT-Bh was expressed as a secreted protein or lipoprotein rather than in the cytop lasm, Oral vaccination with recombinant BCG may be an effective approach, p articularly to induce mucosal IgA and prime for a serum TH1 recall response . (C) 1999 Elsevier Science Ltd. All rights reserved.