HUMAN 5-HT5A RECEPTOR GENE - SYSTEMATIC SCREENING FOR DNA-SEQUENCE VARIATION AND LINKAGE MAPPING ON CHROMOSOME 7Q34-Q36 USING A POLYMORPHISM IN THE 5'-UNTRANSLATED REGION

Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter that media tes a wide range of sensory, motor, and cortical functions by activati ng multiple 5-HT receptor subtypes, In the present study we performed a systematic mutation scan of the complete coding region of the 5-HT5A receptor to explore its variability in the general population. Invest igating 46 unrelated healthy subjects by single-strand conformation an alysis no sequence changes of likely functional relevance were observe d. The detection of a frequent G-->C substitution at]position -19 was used for fine scale linkage mapping of the 5-HT5A gene. Employing a po lymerase-chain-reaction based assay we genotyped 7 CEPN families (Cent re d'Etude du Polymorphisme Humaine) and mapped the receptor to geneti c markers on chromosome 7q34-q36. (C) 1997 Academic Press.