INHIBITION OF SACCHAROMYCES-CEREVISIAE BY COMBINATION OF HYDROSTATIC-PRESSURE AND MONOTERPENES

Using a pressure of 1800 kg/cm, and 150 mu g/mL of alpha-terpinene for 1 hr at 25 degrees C, growth reduction of 6 log cycles after incubati on at 30 degrees C/24h, was found with S. cerevisiae. Without pressuri zation, S. cerevisiae was resistant to 300 mu g/mL and 600 mu g/mL alp ha-terpinene but was sensitive to 1250 mu g/mL. Reduction of 3 log cyc les in viable cell counts was found when cells were exposed to pressur e (1800 kg/cm(2)) and -(+)-limonene (200, 400 and 2,000 mu g/mL).