Ion chromatograph with immobilized alkaline phosphatase reactor for the determination of adenosine phosphates

An ion chromatograph equipped with an immobilized enzyme reactor was develo ped for the determination of adenosine phosphates. The enzyme reactor was p repared by immobilization of alkaline phosphatase onto activated glass bead s. The proposed equipment made it possible to determine adenosine phosphate s rapidly as phosphate ion. Adenosine monophosphate (AMP, 5 x 10(-5)-2 x 10 (-4) M), adenosine diphosphate (ADP, 1 x 10(-5)-1 x 10(-4) M) and adenosine triphosphate (ATP, 1 x 10(-5)-1 x 10(-4) M) could be determined precisely with a relative error of less than 3.6%, in the presence of L-lactic acid, creatine, myoglobin, albumin, chloride, nitrate, phosphate, and sulfate ion s. (C) 1999 Elsevier Science B.V. All rights reserved.