UV-absorbing mycosporine-like compounds in planktonic and benthic organisms from a high-mountain lake

Citation
R. Sommaruga et F. Garcia-pichel, UV-absorbing mycosporine-like compounds in planktonic and benthic organisms from a high-mountain lake, ARCH HYDROB, 144(3), 1999, pp. 255-269
Citations number
41
Categorie Soggetti
Aquatic Sciences
Journal title
ARCHIV FUR HYDROBIOLOGIE
ISSN journal
00039136 → ACNP
Volume
144
Issue
3
Year of publication
1999
Pages
255 - 269
Database
ISI
SICI code
0003-9136(199902)144:3<255:UMCIPA>2.0.ZU;2-I
Abstract
We investigated the occurrence, concentration and composition of mycosporin es (mycosporine-like amino acids, MAAs) in planktonic organisms and epilith ic cyanobacteria from a clear water high-mountain lake situated in the Cent ral Alps, Austria. Two bi-substituted MAAs were identified by HPLC in extra cts made of 1996 plankton samples with 90 % aqueous methanol, i.e. asterina -330 (lambda(max) = 330 mm) and shinorine (lambda(max) = 334 nm). Extracts with 20 % aqueous methanol for 2 h at 45 degrees C revealed the additional presence of another MAA tentatively identified as palythine (lambda(max) = 320 nm) in the 1998 planktonic assemblage. In the upper 3 m of the water co lumn the total concentration of MAAs decreased exponentially with depth, bu t the maxims for both absolute and chlorophyll-a specific concentrations we re observed close to the bottom at 8.5 m depth. This was explained by the a ccumulation of MAAs in the copepod Cyclops abyssorum tatricus that stays in deep water, during daytime. The copepodite III stage contained the 3 MAAs found in phytoplankton but also the mono-substituted compound, mycosporine- glycine (mycosporine-gly: lambda(max) = 310 nm). The concentration of MAAs in C. abyssorum tatricus was highest for shinorine (1.45 % of the dry weigh t) and lowest for mycosporine-gly (0.02 % of the dry weight). Epilithic cya nobacteria had a more diverse MAA spectrum than plankton, and produced not only asterina-330 and shinorine but also palythinol (lambda(max) = 332 nm), my cosporine-gly and two unidentified compounds with lambda(max) = 330 and 340 nm. The composition and also the relative abundance of the cyanobacter ial MAAs changed with depth. Mycosporine-gly was found at the lakeshore whe re Gloeocapsa sp. dominates, but it was absent at 0.5 and 2.5 m depth domin ated by Schizothrix sp. and Tolipothrix sp., respectively. We could not det ect any MAAs in the cysts of the red snow alga Chlamydomonas nivalis, which develops on top of the winter cover shortly before ice-melt. These results expand to alpine lakes the range of ecosystems in which these compounds ma y play a significant biological role.