DEVELOPMENT OF AN ACUTE AND CHRONIC ECOTOXICITY ASSAY USING LUX-MARKED RHIZOBIUM-LEGUMINOSARUM BIOVAR TRIFOLII

Authors
PATON GI PALMER G BURTON M RATTRAY EAS MCGRATH SP GLOVER LA KILLHAM K
Citation
Gi. Paton et al., DEVELOPMENT OF AN ACUTE AND CHRONIC ECOTOXICITY ASSAY USING LUX-MARKED RHIZOBIUM-LEGUMINOSARUM BIOVAR TRIFOLII, Letters in applied microbiology, 24(4), 1997, pp. 296-300
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
24
Issue
4
Year of publication
1997
Pages
296 - 300
Database
ISI
SICI code
0266-8254(1997)24:4<296:DOAAAC>2.0.ZU;2-8
Abstract
A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence . The suitability of the bacterium as a soil pollution biosensor was a ssessed using acute and chronic assays. Bacterial bioluminescence resp onded sensitively to the metals studied, The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlig hted its potential for use as an indicator of soil pollution.