Precise quantitation of human parvovirus B19 DNA in biological samples by PCR

An application of a quantitative PCR-based method was developed for the det ection of human parvovirus B19 DNA. The procedure was characterised accordi ng to guidelines for the validation of analytical procedures. Furthermore, the reliability was demonstrated by the correct quantitation of samples of an international collaborative study. This application might be useful for studies focussed on removal and/or inactivation procedures of human parvovi rus B19 as well as for general screening purposes of biological materials.