Fed-batch production and simple isolation of succinoglycan from Agrobacterium tumefaciens

Succinoglycan production by Agrobacterium tumefaciens was increased from 9. 5 g/l to 14 g/l when the microorganism was cultivated under fed-batch condi tion, as compared to batch fermentation. Cells and broth impurities were re moved from the fermentation broth with activated charcoal at a temperature of 90 degrees C, yielding a clear and odorless solution of the polymer. Vis cosity measurement, NMR spectroscopy and Mw determination proved that the p olymer retains its main features, practically unaffected by the heat treatm ent.