Quantitative recovery of fungal biomass grown on solid kappa-carrageenan media

The use of a dissolvable solid matrix, kappa-carrageenan, to quantify bioma ss grown on solid media was studied. A firm gel was obtained with 2% (w/v) kappa-carrageenan and 20 mM K+ which could be easily dissolved in demineral ized water. Direct quantification of Coniothyrium minitans biomass grown on this medium was feasible. No effects of the dissolution on the amount of b iomass recovered were detected.