USE OF AN AUTOMATIC DNA SEQUENCER FOR S1 MAPPING - TRANSCRIPTIONAL ANALYSIS OF THE STREPTOMYCES-COELICOLOR A3(2) DNAK OPERON

Authors
BRANS A LORIAUX A THAMM I JORIS B DUSART J
Citation
A. Brans et al., USE OF AN AUTOMATIC DNA SEQUENCER FOR S1 MAPPING - TRANSCRIPTIONAL ANALYSIS OF THE STREPTOMYCES-COELICOLOR A3(2) DNAK OPERON, FEMS microbiology letters, 149(2), 1997, pp. 189-194
Citations number
19
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
149
Issue
2
Year of publication
1997
Pages
189 - 194
Database
ISI
SICI code
0378-1097(1997)149:2<189:UOAADS>2.0.ZU;2-V
Abstract
The transcription start point of the dnaK operon of Streptomyces coeli color A3(2) has been determined by SI mapping, using the EMBL automate d fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma(70)-type promoter. This promoter responds to heat shock and invo lves an inverted repeat different from the CIRCE sequence characterist ic of the Gram-positive heat-shock promoters.