Tz. Berardini et al., Identification of conserved promoter elements for aldB and isozyme specific residues in aldolase B, COMP BIOC B, 122(1), 1999, pp. 53-61
Citations number
57
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
The comparison of three complete aldolase B genes-including known and putat
ive regulatory elements-is presented. The third aldolase B gene was provide
d by the complete aldB gene sequence (14803 bp) encoding the rabbit aldolas
e B isozyme. The promoter sequence alignment included the nonmammalian chic
ken aldolase B gene and confirms the promoter sequence conservation of thos
e elements where trans-factor binding has been demonstrated in rat aldB. Mo
reover, the alignment reveals conserved sequences that may represent previo
usly unidentified promoter elements that are present in all aldBs or specif
ically in the mammalian aldB promoters. One remarkable feature is a poly-pu
rine segment found between the CAAT and TATA elements. In the mammalian pro
moters, this is exclusively a 9-10 bp poly-dA stretch. The avian promoter h
as an additional stretch of eight dG-bases immediately upstream of the poly
-dA. Alignment of a portion of intron 1 of the chicken, human, and rabbit a
ldB genes reveals conserved sequences that are likely candidates for a repo
rted positive activation sequence. In addition, the amino acid sequences of
all eight known aldolase B isozymes is compared to the other vertebrate al
dolases. A. number of aldolase B-specific residues are identified that clus
ter in the carboxyl-portion of the sequence. With the exception of residue
C268, these residues are not found near the active site, although, they are
likely to be responsible for the substrate specificity of aldolase B. (C)
1999 Elsevier Science Inc. All rights reserved.