H. Hildebrandt et al., Direct electrospray-ionization mass spectrometric analysis of the major ganglioside from crucian carp liver after thin layer chromatography, COMP BIOC B, 122(1), 1999, pp. 83-88
Citations number
44
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Ganglioside patterns from crucian carp brain, muscle, and liver as well as
liver gangliosides of roach, carp, the cichlid Oreochromis mossambicus, pig
eon, dwarf hamster, and calf were comparatively analyzed by high performanc
e thin layer chromatography (HPTLC). To achieve a rapid estimation on poten
tially interesting ganglioside compounds, electrospray-ionization mass spec
trometry (MS) was directly applied to a chloroform/methanol extract of the
major TLC band of crucian carp liver. The spectrum, obtained from a few mic
rograms of this crude biological sample, revealed a series of peaks corresp
onding to GM4-like monosialoganglioside species. GC-MS analysis revealed hy
droxylated fatty acids ranging from 2 h 20 min:0 to 2 h 26 min:0 for the [M
'H](-) ions of m/z 1061-1145. Collision induced dissociation tandem MS/MS o
f the major peak with a [M'H] ion of m/z 1117 demonstrated the presence of
N-acetylneuraminic acid as sialic acid compound. The sugar composition was
confirmed by GLC as galactose and sialic acid in a 1:1 molar ratio. Thus, t
he structure of the ion at mit 1117 is N-acetylneuraminylgalactosylceramide
(NeuAc-Gal-Cer) with the long chain base d18:1 and the hydroxylated fatty
acid 2 h 24 min:0. The results demonstrate for the first time unambiguously
that NeuAc-Gal-Cer is the main ganglioside fraction in fish liver and that
electrospray ionization-mass spectrometry (ESI-MS) can be used to elucidat
e the chemical composition of a ganglioside fraction obtained by convenient
extraction of a HPTLC band. (C) 1999 Elsevier Science Inc. All rights rese
rved.